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goat anti mouse il 4 (R&D Systems)

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R&D Systems goat anti mouse il 4
Goat Anti Mouse Il 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+mouse+il+4/Goat+Anti-Mouse+IgM+PE-conjugated+Antibody/pmc05425473-61-45-47
Average 93 stars, based on 339 article reviews

goat anti mouse il 4 - by Bioz Stars, 2026-09

93/100 stars

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Article Title: Automated analysis of two- and three-color fluorescent Elispot (Fluorospot) assays for cytokine secretion
Article Snippet: Anti-mouse MIP-1α (39624.11), goat anti-mouse IL-4 (Cat. AF-404-NA), goat anti-MIP-1α (Cat. AF-450-NA) were all obtained from R&D Systems (Minneapolis, MN).

Incubation:

Article Title: Role of CD4 + T helper 2-type cells in cutaneous inflammatory responses induced by fluorescein isothiocyanate
Article Snippet: Recombinant murine IL-4 (specific activity 1–2 × 10 7 U/mg; Genzyme) diluted in RPMI-FCS was added to triplicate wells and samples of conditioned medium diluted to different extents in RPMI-FCS were added to duplicate wells and the plates incubated for 2 hr at room temperature. .. Plates were then incubated for 2 hr at room temperature with 8 μg/ml of goat anti-mouse IL-4 (R & D Systems Europe, Abingdon, Oxon, UK) diluted in RPMI-FCS, followed by a further 2-hr incubation at room temperature with a 1 : 1000 dilution in RPMI-FCS of peroxidase-conjugated donkey anti-goat IgG (Serotec). ..

Article Title: Cytokine Responses Induced by Skin Exposure of Mice to Chemical Allergens: Role of Interleukin 12
Article Snippet: We reported previously that prolonged (13 day) topical exposure to chemical contact and respiratory allergens such as 2,4-dinitrochlorobenzene (DNCB) and trimellitic anhydride (TMA) results in cytokine phenotypes consistent with the selective activation of type 1 and type 2 cells, respectively.. In the current experiments, the role of the type 1 inducing heterodimeric cytokine interleukin (IL)-12 in the development of these divergent cytokine secretion phenotypes has been examined by using an enzyme-linked immunosorbant assay and neutralizing antibody specific for the p40 subunit of the dimer.. After acute exposure, when a mixed Th0 phenotype is observed following treatment with allergen, both DNCBand TMA-activated lymph node cells (LNC) secrete similar levels of interferon-g (IFN-g) and IL-12 p40, and neutralizing anti-IL-12 p40 antibody profoundly inhibits expression of IFN-g by both DNCBand TMA-stimulated LNC.

Recombinant:

Article Title: Direct Binding of the pH-Regulated Protein 1 (Pra1) from Candida albicans Inhibits Cytokine Secretion by Mouse CD4 + T Cells
Article Snippet: To generate Th1 cells pooled spleen/lymph node cells from an OT-II mouse were depleted of CD4 + CD25 + regulatory T cells using anti-CD25 biotin (5 μg/ml, BD) and Streptavidin-beads and passage over an LD column (both Miltenyi). .. CD25-depleted spleen/lymph node cells were then seeded at 1 × 10 6 cells/well (48-well plate, Greiner, final volume: 1 ml, 3 × 10 6 cells in total), OVAp was added at 1 μM, recombinant mouse IL-12 (R&D Systems, Minneapolis, MN, USA) at 10 ng/ml and goat-anti-mouse IL-4 (R&D Systems) at 10 μg/ml. ..